8G9X

Cryo-EM structure of vFP49.02 Fab in complex with HIV-1 Env BG505 DS-SOSIP.664 (conformation 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, D, O	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S6) Pfam (PF00517) In PDB	Human immunodeficiency virus 1	Env polyprotein
2	E, G, P	Envelope glycoprotein gp120	polymer	481	54086.3	3	UniProt (Q2N0S6) Pfam (PF00516) In PDB	Human immunodeficiency virus 1	Env polyprotein
3	H	vFP49.02 heavy chain	polymer	235	25020.0	1		Mus musculus
4	L	vFP49.02 light chain	polymer	214	23621.0	1		Mus musculus
5	b, c, d, h, A...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	24	In PDB GlyTouCan (G42666HT)
6	e, f, g, C, j...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	12	In PDB GlyTouCan (G15407YE)
7	a, K, n	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
8	E, G, O, P, B...	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	15	Chemie (NAG)

Sequence modifications

B, D, O: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict

E, G, P: 31 - 513 (UniProt: Q2N0S6)

PDB	External Database	Details
Cys 201	Ile 200	conflict
Asn 332	Thr 330	conflict
Cys 433	Ala 430	conflict
Cys 501	Ala 498	conflict
Arg 509	Glu 506	conflict
Arg 510	Lys 507	conflict
Arg 512	Ala 509	conflict
Arg 513	Val 510	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	262339.4
Branched	Number of molecules	39
	Total formula weight	19470.2
Non-Polymers*	Number of molecules	15
	Total formula weight	3318.1
All*	Total formula weight	285127.7

*Water molecules are not included.