8G9U
Exploiting Activation and Inactivation Mechanisms in Type I-C CRISPR-Cas3 for Genome Editing Applications
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | Cas3 | polymer | 771 | 84572.8 | 1 | UniProt (by SIFTS) (D0W8X3) | Neisseria lactamica | |
| 2 | B, C, D, E, F... (A, B, D, C, E...) | CRISPR-associated protein, Csd2 family | polymer | 283 | 32208.1 | 7 | UniProt (D0W8X6) | Neisseria lactamica | |
| 3 | H, I, K (I, G, J) | Phage associated protein | polymer | 124 | 14245.2 | 3 | UniProt (by SIFTS) (D0W8X5) | Neisseria lactamica | |
| 4 | J (H) | CRISPR-associated protein, Csd1 family | polymer | 582 | 64718.6 | 1 | UniProt (D0W8X5) | Neisseria lactamica | |
| 5 | L (K) | crRNA (43-MER) | polymer | 43 | 13816.2 | 1 | Neisseria lactamica | ||
| 6 | M (L) | Traget strand DNA (53-MER) | polymer | 53 | 16410.5 | 1 | GenBank (1394359882) | Neisseria lactamica | |
| 7 | O (N) | pre-crRNA processing endonuclease | polymer | 205 | 23870.5 | 1 | UniProt (D0W8X4) | Neisseria lactamica | |
| 8 | P (O) | proximal nontarget strand DNA (5'-D(P*AP*TP*GP*AP*AP*CP*TP*TP*CP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') | polymer | 20 | 6160.1 | 1 | Neisseria lactamica | ||
| 9 | Q (Q) | Distal nontarget DNA (5'-D(P*TP*TP*AP*TP*AP*TP*TP*AP*AP*TP*AP*TP*TP*AP*TP*AP*TP*TP*TP*A)-3') | polymer | 20 | 6111.0 | 1 | Neisseria lactamica | ||
| 10 | R, S (P) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | T, U, V, W (P, O, Q) | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) |
Sequence modifications
A, B, D, C, E, F, M: 2 - 283 (UniProt: D0W8X6)
H: 1 - 582 (UniProt: D0W8X5)
L: 9 - 59 (GenBank: 1394359882)
| PDB | External Database | Details |
|---|---|---|
| Gly 284 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 190 | Val 190 | conflict |
| Ala 239 | Ile 239 | conflict |
| Ile 242 | Val 242 | conflict |
| Gly 260 | Ser 260 | conflict |
| Thr 271 | Ala 271 | conflict |
| Asn 296 | Lys 296 | conflict |
| Ala 299 | Glu 299 | conflict |
| Ala 306 | Thr 306 | conflict |
| Cys 317 | Gln 317 | conflict |
| Glu 322 | Lys 322 | conflict |
| Asp 323 | Glu 323 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Da 7 | - | expression tag |
| Dg 8 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 17 |
| Total formula weight | 483851.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 428.5 | |
| All* | Total formula weight | 484280.4 |
