8G93

Crystal structures of 17-beta-hydroxysteroid dehydrogenase 13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Hydroxysteroid 17-beta dehydrogenase 13	polymer	315	35350.1	2	UniProt (A0A8C0PP93) Pfam (PF00106) UniProt (by SIFTS) (A0A8I3RYU0)	Canis lupus familiaris (dog)	Hydroxysteroid 17-beta dehydrogenase 11
2	C, E (A, B)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3	D, F (A, B)	3-fluoro-N-({(1r,4r)-4-[(2-fluorophenoxy)methyl]-1-hydroxycyclohexyl}methyl)-4-hydroxybenzamide	non-polymer		391.4	2	Chemie (YXW)
4	G, H (A, B)	water	water		18.0	73	Chemie (HOH)

Sequence modifications

A, B: 2 - 300 (UniProt: A0A8C0PP93)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	cloning artifact
Glu 177	Gly 177	engineered mutation
Gly 178	Val 178	engineered mutation
Ala 205	Thr 205	engineered mutation
Val 293	Ile 293	engineered mutation
Gly 301	-	expression tag
Ser 302	-	expression tag
Gly 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70700.3
Non-Polymers*	Number of molecules	4
	Total formula weight	2109.7
All*	Total formula weight	72809.9

*Water molecules are not included.