8G8Z

Cryo-EM structure of 3DVA component 1 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (39-MER)	polymer	39	12063.8	1		Escherichia coli
2	B (B)	DNA (31-MER)	polymer	31	9486.1	1		Escherichia coli
3	C, D (G, H)	DNA-directed RNA polymerase subunit alpha	polymer	235	25971.5	2	UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4)	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	E (K)	DNA-directed RNA polymerase subunit omega	polymer	80	9094.2	1	UniProt (A0A1X3IVJ5) Pfam (PF01192) UniProt (by SIFTS) (P0A800)	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (R)	RNA (48-MER)	polymer	48	15404.3	1		Escherichia coli
6	G (I)	DNA-directed RNA polymerase subunit beta	polymer	1340	150560.6	1	UniProt (C3SIA7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2)	Escherichia coli
7	H (J)	DNA-directed RNA polymerase subunit beta'	polymer	1358	150436.3	1	UniProt (A0A369F490) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7)	Escherichia coli
8	I (R)	7-DEAZA-7-AMINOMETHYL-GUANINE	non-polymer		179.2	1	Chemie (PRF)
9	J (J)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

G, H: 1 - 234 (UniProt: A0A5B9AW69)

PDB	External Database	Details
Glu 235	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	398988.3
Non-Polymers*	Number of molecules	2
	Total formula weight	203.5
All*	Total formula weight	399191.8

*Water molecules are not included.