8G7E
Cryo-EM structure of 3DVA component 0 of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (39-mer) | polymer | 39 | 12063.8 | 1 | Escherichia coli | ||
| 2 | B (B) | DNA (31-MER) | polymer | 31 | 9486.1 | 1 | Escherichia coli | ||
| 3 | C, D (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 235 | 25971.5 | 2 | UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 79 | 8963.0 | 1 | UniProt (L4IY67) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | |
| 5 | F (R) | RNA (47-MER) | polymer | 47 | 15059.1 | 1 | Escherichia coli | ||
| 6 | G (I) | DNA-directed RNA polymerase subunit beta | polymer | 1341 | 150691.8 | 1 | UniProt (A7ZUK1) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | |
| 7 | H (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1434 | 158571.2 | 1 | UniProt (A7ZUK2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 8 | I (R) | 7-DEAZA-7-AMINOMETHYL-GUANINE | non-polymer | 179.2 | 1 | Chemie (PRF) | |||
| 9 | J (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
G, H: 1 - 234 (UniProt: A0A5B9AW69)
J: 1 - 1407 (UniProt: A7ZUK2)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Val 1 | Met 1 | conflict |
| Leu 1408 | - | expression tag |
| Asp 1409 | - | expression tag |
| Arg 1410 | - | expression tag |
| Arg 1411 | - | expression tag |
| Ala 1412 | - | expression tag |
| Ser 1413 | - | expression tag |
| Glu 1414 | - | expression tag |
| Asn 1415 | - | expression tag |
| Leu 1416 | - | expression tag |
| Tyr 1417 | - | expression tag |
| Phe 1418 | - | expression tag |
| Gln 1419 | - | expression tag |
| Gly 1420 | - | expression tag |
| Gly 1421 | - | expression tag |
| Leu 1422 | - | expression tag |
| Asn 1423 | - | expression tag |
| Asp 1424 | - | expression tag |
| Ile 1425 | - | expression tag |
| Phe 1426 | - | expression tag |
| Glu 1427 | - | expression tag |
| Ala 1428 | - | expression tag |
| Gln 1429 | - | expression tag |
| Lys 1430 | - | expression tag |
| Ile 1431 | - | expression tag |
| Glu 1432 | - | expression tag |
| Trp 1433 | - | expression tag |
| His 1434 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 406778.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 203.5 | |
| All* | Total formula weight | 406981.5 |
