8G4W

Cryo-EM consensus structure of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase plus preQ1 ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA (39-mer)	polymer	39	12063.8	1		Escherichia coli
2	B	DNA (31-MER)	polymer	31	9486.1	1		Escherichia coli
3	G, H	DNA-directed RNA polymerase subunit alpha	polymer	235	25971.5	2	UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	K	DNA-directed RNA polymerase subunit omega	polymer	79	8963.0	1	UniProt (A1AHI0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	R	RNA (47-MER)	polymer	47	15059.1	1		Escherichia coli
6	I	DNA-directed RNA polymerase subunit beta	polymer	1340	150560.6	1	UniProt (C3SIA7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli
7	J	DNA-directed RNA polymerase subunit beta'	polymer	1358	150436.3	1	UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) In PDB	Escherichia coli
8	R	7-DEAZA-7-AMINOMETHYL-GUANINE	non-polymer		179.2	1	Chemie (PRF)
9	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

G, H: 1 - 234 (UniProt: A0A5B9AW69)

PDB	External Database	Details
Glu 235	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	398511.9
Non-Polymers*	Number of molecules	2
	Total formula weight	203.5
All*	Total formula weight	398715.4

*Water molecules are not included.