8G4H
KRAS G13C complex with GDP imaged on a cryo-EM imaging scaffold
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RCG-33 - Cryo-EM imaging scaffold subunit B fused to DARPin | polymer | 321 | 35452.4 | 1 | synthetic construct | ||
| 2 | B (B) | GTPase KRas | polymer | 171 | 19439.9 | 1 | UniProt (P01116) Pfam (PF00071) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 3 | C (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 4 | D (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
B: 1 - 169 (UniProt: P01116)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Cys 13 | Gly 13 | engineered mutation |
| Ser 51 | Cys 51 | conflict |
| Leu 80 | Cys 80 | conflict |
| Ser 118 | Cys 118 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 54892.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 467.5 | |
| All* | Total formula weight | 55359.8 |
