8G4F
KRAS G12V complex with GDP imaged on a cryo-EM imaging scaffold
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RCG-33 - Cryo-EM imaging scaffold subunit B fused to DARPin | polymer | 321 | 35452.4 | 1 | synthetic construct | ||
| 2 | B (B) | GTPase KRas | polymer | 169 | 19208.6 | 1 | UniProt (P01116) Pfam (PF00071) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 3 | C (B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 4 | D (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (B) | water | water | 18.0 | 3 | Chemie (HOH) |
Sequence modifications
B: 1 - 166 (UniProt: P01116)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Val 12 | Gly 12 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 54661.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 467.5 | |
| All* | Total formula weight | 55128.6 |
