8G42

KRAS G12C complex with GDP imaged on a cryo-EM imaging scaffold

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RCG-33 - Cryo-EM imaging scaffold subunit B fused to DARPin	polymer	321	35452.4	1		synthetic construct
2	B	GTPase KRas	polymer	189	21622.3	1	UniProt (P01116) Pfam (PF00071) In PDB	Homo sapiens (human)	K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras
3	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
4	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

B: 1 - 169 (UniProt: P01116)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag
Cys 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57074.7
Non-Polymers*	Number of molecules	2
	Total formula weight	467.5
All*	Total formula weight	57542.2

*Water molecules are not included.