8G3K

Cryo-EM imaging scaffold subunits A and B used to display KRAS G12C complex with GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cob_adeno_trans domain-containing protein	polymer	178	20173.4	1	UniProt (O58404)	Thermoplasma acidophilum
2	B (B)	Cryo-EM imaging scaffold subunit B with DARPin - RCG-33	polymer	321	35452.4	1		synthetic construct

Sequence modifications

A: 1 - 171 (UniProt: O58404)

PDB	External Database	Details
Asp 25	Ser 25	conflict
Ala 88	Glu 88	conflict
Glu 92	Ser 92	conflict
Ser 95	Glu 95	conflict
Lys 97	Leu 100	conflict
Leu 97	Lys 101	conflict
Thr 139	Lys 140	conflict
Leu 140	Glu 141	conflict
Ala 142	Leu 143	conflict
Ile 143	Val 144	conflict
Ala 146	Asn 147	conflict
Leu 147	Arg 148	conflict
Arg 150	Asp 151	conflict
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55625.8
All*	Total formula weight	55625.8

*Water molecules are not included.