8G0I
High Affinity nanobodies against GFP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein | polymer | 255 | 28794.4 | 1 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 2 | B (D) | LaG24 Nanobody | polymer | 137 | 14780.5 | 1 | Lama glama | ||
| 3 | C, D (A, D) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 4 | E, F (D) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | G, H (A, D) | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
A: 2 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ala 1 | - | expression tag |
| Cr2 66 | Ser 65 | chromophore |
| Cr2 66 | Tyr 66 | chromophore |
| Cr2 66 | Gly 67 | chromophore |
| Ala 72 | Ser 72 | conflict |
| His 177 | Gln 177 | conflict |
| Gly 239 | - | expression tag |
| Leu 240 | - | expression tag |
| Glu 241 | - | expression tag |
| Val 242 | - | expression tag |
| Leu 243 | - | expression tag |
| Phe 244 | - | expression tag |
| Gln 245 | - | expression tag |
| Gly 246 | - | expression tag |
| Pro 247 | - | expression tag |
| Ser 248 | - | expression tag |
| His 249 | - | expression tag |
| His 250 | - | expression tag |
| His 251 | - | expression tag |
| His 252 | - | expression tag |
| His 253 | - | expression tag |
| His 254 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 43574.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 116.9 | |
| All* | Total formula weight | 43691.7 |
