8G0I

High Affinity nanobodies against GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	255	28794.4	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria
2	D	LaG24 Nanobody	polymer	137	14780.5	1		Lama glama
3	A, D	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	D	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5		water	water		18.0	66	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 72	Ser 72	conflict
His 177	Gln 177	conflict
Gly 239	-	expression tag
Leu 240	-	expression tag
Glu 241	-	expression tag
Val 242	-	expression tag
Leu 243	-	expression tag
Phe 244	-	expression tag
Gln 245	-	expression tag
Gly 246	-	expression tag
Pro 247	-	expression tag
Ser 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43574.9
Non-Polymers*	Number of molecules	4
	Total formula weight	116.9
All*	Total formula weight	43691.7

*Water molecules are not included.