8FZT

SpCas9 with dual-guide RNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	E	CRISPR-associated endonuclease Cas9/Csn1	polymer	1384	160774.3	1	UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB	Streptococcus pyogenes	SpCas9,SpyCas9
2	A	CrE2	polymer	42	13370.9	1		Streptococcus pyogenes
3	B	tracrRNA	polymer	70	22574.5	1	GenBank (1403816625)	Streptococcus pyogenes
4	C	E2_TS	polymer	40	12334.9	1		Homo sapiens
5	D	E2-NTS	polymer	40	12290.9	1		Homo sapiens

Sequence modifications

E: 1 - 1368 (UniProt: Q99ZW2)

PDB	External Database	Details
Pro 1369	-	expression tag
Lys 1370	-	expression tag
Lys 1371	-	expression tag
Lys 1372	-	expression tag
Arg 1373	-	expression tag
Lys 1374	-	expression tag
Val 1375	-	expression tag
Met 1376	-	expression tag
Asp 1377	-	expression tag
Lys 1378	-	expression tag
His 1379	-	expression tag
His 1380	-	expression tag
His 1381	-	expression tag
His 1382	-	expression tag
His 1383	-	expression tag
His 1384	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	221345.5
All*	Total formula weight	221345.5

*Water molecules are not included.