8FZT
SpCas9 with dual-guide RNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1384 | 160774.3 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (A) | CrE2 | polymer | 42 | 13370.9 | 1 | Streptococcus pyogenes | ||
| 3 | C (B) | tracrRNA | polymer | 70 | 22574.5 | 1 | GenBank (1403816625) | Streptococcus pyogenes | |
| 4 | D (C) | E2_TS | polymer | 40 | 12334.9 | 1 | Homo sapiens | ||
| 5 | E (D) | E2-NTS | polymer | 40 | 12290.9 | 1 | Homo sapiens |
Sequence modifications
E: 1 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Pro 1369 | - | expression tag |
| Lys 1370 | - | expression tag |
| Lys 1371 | - | expression tag |
| Lys 1372 | - | expression tag |
| Arg 1373 | - | expression tag |
| Lys 1374 | - | expression tag |
| Val 1375 | - | expression tag |
| Met 1376 | - | expression tag |
| Asp 1377 | - | expression tag |
| Lys 1378 | - | expression tag |
| His 1379 | - | expression tag |
| His 1380 | - | expression tag |
| His 1381 | - | expression tag |
| His 1382 | - | expression tag |
| His 1383 | - | expression tag |
| His 1384 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 221345.5 | |
| All* | Total formula weight | 221345.5 |
