8FYJ

Structure of HIV-1 BG505 SOSIP-HT2 in complex with two CD4 molecules (class I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Envelope glycoprotein gp160	polymer	474	53193.2	2	UniProt (Q2N0S5) Pfam (PF00516) UniProt (by SIFTS) (Q2N0S6) In PDB	Human immunodeficiency virus 1	Env polyprotein
2	C	Envelope glycoprotein gp160	polymer	474	53235.4	1	UniProt (Q2N0S5) Pfam (PF00516) In PDB	Human immunodeficiency virus 1	Env polyprotein
3	D, E, F	Envelope glycoprotein gp41	polymer	153	17146.5	3	UniProt (Q2N0S6) Pfam (PF00517) In PDB	Human immunodeficiency virus 1	Env polyprotein
4	Y, Z	T-cell surface glycoprotein CD4	polymer	379	42325.5	2	UniProt (P01730) Pfam (PF00047) Pfam (PF05790) Pfam (PF09191) In PDB	Homo sapiens (human)	T-cell surface antigen T4/Leu-3
5	G, J, N	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	3	In PDB GlyTouCan (G81315DD)
6	H	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
7	I, L, M, O	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
8	K	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
9	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)

Sequence modifications

A, B: 33 - 508 (UniProt: Q2N0S5)

PDB	External Database	Details
Trp 315	Ala 313	conflict
Asn 332	Thr 330	conflict
Cys 501	Ala 498	conflict

C: 33 - 508 (UniProt: Q2N0S5)

PDB	External Database	Details
Trp 316	Ala 313	conflict
Asn 332	Thr 330	conflict
Arg 368	Asp 366	engineered mutation
Cys 501	Ala 498	conflict

D, E, F: 512 - 664 (UniProt: Q2N0S6)

PDB	External Database	Details
Pro 559	Ile 556	conflict
Cys 605	Thr 602	conflict

Y, Z: 1 - 371 (UniProt: P01730)

PDB	External Database	Details
Gly 372	-	expression tag
Ser 373	-	expression tag
His 374	-	expression tag
His 375	-	expression tag
His 376	-	expression tag
His 377	-	expression tag
His 378	-	expression tag
His 379	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	295712.2
Branched	Number of molecules	9
	Total formula weight	5278.9
Non-Polymers*	Number of molecules	7
	Total formula weight	1548.5
All*	Total formula weight	302539.5

*Water molecules are not included.