8FWJ

Structure of dodecameric KaiC-RS-S413E/S414E complexed with KaiB-RS solved by cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Circadian clock protein KaiC	polymer	568	62666.1	12	UniProt (B9KWX8) Pfam (PF06745) In PDB	Cereibacter sphaeroides
2	M, N, O, P, Q...	Circadian clock protein KaiB	polymer	92	10317.1	12	UniProt (A0A3G6WWB7) In PDB	Cereibacter sphaeroides
3	H, B, I, C, J...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	12	Chemie (ATP)
4	H, B, I, C, J...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	12	Chemie (ADP)
5	A, H, B, I, C...	MAGNESIUM ION	non-polymer		24.3	24	Chemie (MG)
6		water	water		18.0	48	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 566 (UniProt: B9KWX8)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Glu 413	Ser 413	engineered mutation
Glu 414	Ser 414	engineered mutation

M, N, O, P, Q, R, S, T, U, V, W, X: 3 - 90 (UniProt: A0A3G6WWB7)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Gly 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	875799.1
Non-Polymers*	Number of molecules	48
	Total formula weight	11795.9
All*	Total formula weight	887595.0

*Water molecules are not included.