8FWI

Structure of dodecameric KaiC-RS-S413E/S414E solved by cryo-EM

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Circadian clock protein KaiC	polymer	568	62666.1	12	UniProt (B9KWX8) Pfam (PF06745) In PDB	Cereibacter sphaeroides
2	K, E, L, F, G...	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	12	Chemie (ATP)
3	K, E, L, F, G...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	12	Chemie (ADP)
4	D, K, E, L, F...	MAGNESIUM ION	non-polymer		24.3	24	Chemie (MG)
5		water	water		18.0	72	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 566 (UniProt: B9KWX8)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Glu 413	Ser 413	engineered mutation
Glu 414	Ser 414	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	751993.6
Non-Polymers*	Number of molecules	48
	Total formula weight	11795.9
All*	Total formula weight	763789.5

*Water molecules are not included.