8FTJ

Crystal structure of human NEIL1 (P2G (242K) C(delta)100) glycosylase bound to DNA duplex containing urea

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endonuclease 8-like 1	polymer	297	33641.4	1	UniProt (Q96FI4) Pfam (PF01149) Pfam (PF09292) In PDB	Homo sapiens (human)	DNA glycosylase/AP lyase Neil1,DNA-(apurinic or apyrimidinic site) lyase Neil1,Endonuclease VIII-like 1,FPG1,Nei homolog 1,NEH1,Nei-like protein 1
2	D	DNA (5'-D(*CP*GP*TP*CP*CP*AP*UDV*GP*TP*CP*TP*AP*CP)-3')	polymer	13	3838.5	1		synthetic construct
3	F	DNA (5'-D(*TP*AP*GP*AP*CP*AP*TP*GP*GP*AP*CP*GP*G)-3')	polymer	13	4040.6	1		synthetic construct
4	A	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
5	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6		water	water		18.0	127	Chemie (HOH)

Sequence modifications

A: 2 - 290 (UniProt: Q96FI4)

PDB	External Database	Details
Gly 2	Pro 2	engineered mutation
Leu 291	-	expression tag
Glu 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41520.6
Non-Polymers*	Number of molecules	6
	Total formula weight	474.4
All*	Total formula weight	41995.0

*Water molecules are not included.