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8FS7

Structure of S. cerevisiae Rad24-RFC loading the 9-1-1 clamp onto a 10-nt gapped DNA in step 5 (closed 9-1-1 and stably bound chamber DNA)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Checkpoint protein RAD24polymer54562792.51UniProt (P32641)
Pfam (PF03215)
Saccharomyces cerevisiae (baker's yeast)
2B
(B)
Replication factor C subunit 4polymer32336201.01UniProt (P40339)
Pfam (PF00004)
Pfam (PF08542)
Saccharomyces cerevisiae (baker's yeast)Replication factor C4,Activator 1 37 kDa subunit
3C
(C)
Replication factor C subunit 3polymer33637841.11UniProt (P38629)
Pfam (PF00004)
Pfam (PF08542)
Saccharomyces cerevisiae (baker's yeast)Replication factor C3,Activator 1 40 kDa subunit
4D
(D)
Replication factor C subunit 2polymer35339794.51UniProt (P40348)
Pfam (PF00004)
Pfam (PF21960)
Pfam (PF08542)
Saccharomyces cerevisiae (baker's yeast)Replication factor C2,Activator 1 41 kDa subunit
5E
(E)
Replication factor C subunit 5polymer35439993.61UniProt (P38251)
Pfam (PF21960)
Pfam (PF22534)
Pfam (PF13177)
Saccharomyces cerevisiae (baker's yeast)Replication factor C5,Activator 1 40 kDa subunit
6F
(F)
DNA damage checkpoint control protein MEC3polymer47453207.81UniProt (Q02574)
Pfam (PF04005)
Saccharomyces cerevisiae (baker's yeast)
7G
(G)
DNA damage checkpoint control protein RAD17polymer40145637.51UniProt (A0A8H4BW58)
Pfam (PF02144)
UniProt (by SIFTS) (P48581)
Saccharomyces cerevisiae (baker's yeast)
8H
(H)
DDC1 isoform 1polymer61269787.51UniProt (A0A8H4BUG7)
UniProt (by SIFTS) (Q08949)
Saccharomyces cerevisiae (baker's yeast)
9I
(I)
Template strandpolymer5015389.91synthetic construct
10J
(J)
Primer strand 1polymer216464.21synthetic construct
11K
(K)
Primer strand 2polymer206084.91synthetic construct
12L, O, P, S
(A, B, C, D)
MAGNESIUM IONnon-polymer24.34Chemie (MG)
13M, N, Q, R
(A, B, C, D)
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTERnon-polymer523.24Chemie (AGS)
14T
(E)
ADENOSINE-5'-DIPHOSPHATEnon-polymer427.21Chemie (ADP)
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains11
Total formula weight413194.4
Non-Polymers*Number of molecules9
Total formula weight2617.4
All*Total formula weight415811.8
*Water molecules are not included.

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PDB entries from 2025-05-28

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