8FQB

GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 10mM CaCl2, 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Ca10)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glutamate receptor 2	polymer	889	99559.5	4	UniProt (P19491) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Norway rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	E, F, G, H (E, G, F, H)	Voltage-dependent calcium channel gamma-2 subunit	polymer	336	37109.8	4	UniProt (O88602) Pfam (PF00822)	Mus musculus (house mouse)	Neuronal voltage-gated calcium channel gamma-2 subunit,Stargazin,Transmembrane AMPAR regulatory protein gamma-2,TARP gamma-2
3	I (C)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4	J, K (F, H)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	L, M, N, O, P... (A, B, C, D, E...)	water	water		18.0	176	Chemie (HOH)

Sequence modifications

A, B, C, D: -20 - 868 (UniProt: P19491)

PDB	External Database	Details
Asp 848	-	insertion
Tyr 849	-	insertion
Lys 850	-	insertion
Asp 851	-	insertion
Asp 852	-	insertion
Asp 853	-	insertion
Asp 854	Tyr 869	conflict

E, G, F, H: 1 - 323 (UniProt: O88602)

PDB	External Database	Details
Glu 52	Lys 52	engineered mutation
Glu 53	Lys 53	engineered mutation
Gly 324	-	expression tag
Gly 325	-	expression tag
Arg 326	-	expression tag
Gly 327	-	expression tag
Gly 328	-	expression tag
Thr 329	-	expression tag
Glu 330	-	expression tag
Thr 331	-	expression tag
Ser 332	-	expression tag
Gln 333	-	expression tag
Ala 334	-	expression tag
Pro 335	-	expression tag
Ala 336	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	546677.1
Non-Polymers*	Number of molecules	3
	Total formula weight	111.0
All*	Total formula weight	546788.1

*Water molecules are not included.