8FQ5

GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma2, with 140mM NMDG, 330uM CTZ, and 100mM L-glutamate (Open-Na110)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Glutamate receptor 2	polymer	889	99559.5	4	UniProt (P19491) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) In PDB	Rattus norvegicus (Norway rat)	GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2
2	E, G, F, H	Voltage-dependent calcium channel gamma-2 subunit	polymer	336	37109.8	4	UniProt (O88602) Pfam (PF00822) In PDB	Mus musculus (house mouse)	Neuronal voltage-gated calcium channel gamma-2 subunit,Stargazin,Transmembrane AMPAR regulatory protein gamma-2,TARP gamma-2
3	E, G, F, H	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
4		water	water		18.0	220	Chemie (HOH)

Sequence modifications

A, B, C, D: -20 - 868 (UniProt: P19491)

PDB	External Database	Details
Asp 848	Tyr 869	engineered mutation
Tyr 849	-	insertion
Lys 850	-	insertion
Asp 851	-	insertion
Asp 852	-	insertion
Asp 853	-	insertion
Asp 854	-	insertion

E, G, F, H: 1 - 323 (UniProt: O88602)

PDB	External Database	Details
Glu 52	Lys 52	engineered mutation
Glu 53	Lys 53	engineered mutation
Gly 324	-	expression tag
Gly 325	-	expression tag
Arg 326	-	expression tag
Gly 327	-	expression tag
Gly 328	-	expression tag
Thr 329	-	expression tag
Glu 330	-	expression tag
Thr 331	-	expression tag
Ser 332	-	expression tag
Gln 333	-	expression tag
Ala 334	-	expression tag
Pro 335	-	expression tag
Ala 336	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	546677.1
Non-Polymers*	Number of molecules	4
	Total formula weight	141.8
All*	Total formula weight	546818.9

*Water molecules are not included.