8FQ3
LBD conformation 2 (LBDconf2) of GluA2 flip Q isoform N619K mutant of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg/N619K)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (B, C, D, A) | Glutamate receptor 2 | polymer | 889 | 99574.5 | 4 | UniProt (P19491) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060) | Rattus norvegicus (Norway rat) | GluR-2,AMPA-selective glutamate receptor 2,GluR-B,GluR-K2,Glutamate receptor ionotropic,AMPA 2,GluA2 |
| 2 | E, G, I, K (B, C, D, A) | GLUTAMIC ACID | non-polymer | 147.1 | 4 | Chemie (GLU) PubChem (611) PubChem (23327) PubChem (33032) | |||
| 3 | F, H, J, L (B, C, D, A) | CYCLOTHIAZIDE | non-polymer | 389.9 | 4 | Chemie (CYZ) PubChem (2910) PubChem (21550932) PubChem (177678892) PubChem (44582559) PubChem (52949708) PubChem (657321) PubChem (5288009) PubChem (92142359) PubChem (98047041) PubChem (101691639) PubChem (5388988) PubChem (92143401) PubChem (92215537) PubChem (6603794) PubChem (98047042) PubChem (101691640) PubChem (11972587) PubChem (131842006) | |||
| 4 | M, N, O, P (B, C, D, A) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
B, C, D, A: -20 - 868 (UniProt: P19491)
| PDB | External Database | Details |
|---|---|---|
| Lys 619 | Asn 640 | engineered mutation |
| Asp 848 | - | insertion |
| Tyr 849 | - | insertion |
| Lys 850 | - | insertion |
| Asp 851 | - | insertion |
| Asp 852 | - | insertion |
| Asp 853 | - | insertion |
| Asp 854 | Tyr 869 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 398298.2 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2148.0 | |
| All* | Total formula weight | 400446.2 |
