8FOL

The structure of a crystallizable variant of E. coli pyruvate formate-lyase activating enzyme bound to SAM, alternate crystal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pyruvate formate-lyase 1-activating enzyme	polymer	245	28237.3	1	UniProt (P0A9N4) Pfam (PF13353) Pfam (PF04055) In PDB	Escherichia coli K-12	Formate-C-acetyltransferase-activating enzyme 1,PFL-activating enzyme 1
2	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
3	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
4	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
6		water	water		18.0	10	Chemie (HOH)

Sequence modifications

A: 1 - 245 (UniProt: P0A9N4)

PDB	External Database	Details
Glu 1	Ser 2	engineered mutation
Lys 53	Glu 54	engineered mutation
Glu 93	Ala 94	engineered mutation
His 111	Arg 112	engineered mutation
Lys 139	Gln 140	engineered mutation
Arg 151	Glu 152	engineered mutation
Gln 154	Lys 155	engineered mutation
Glu 158	Asn 159	engineered mutation
Glu 222	Lys 223	engineered mutation
Arg 225	Lys 226	engineered mutation
Ala 226	Lys 227	engineered mutation
Arg 230	Glu 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28237.3
Non-Polymers*	Number of molecules	6
	Total formula weight	895.5
All*	Total formula weight	29132.8

*Water molecules are not included.