8FF5

Cryo-EM structure of Cascade-DNA-fullRloop in type I-B CAST system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A	polymer	212	24852.9	1	UniProt (A0A235IG00) In PDB	Peltigera membranacea
2	B	Type I-B CRISPR-associated protein Cas6	polymer	220	24945.7	1	UniProt (A0A235IH92) Pfam (PF09559) In PDB	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
3	C, D, E, F, G...	Type I-B CRISPR-associated protein Cas7	polymer	323	37299.0	6	UniProt (A0A235IG15) In PDB	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
4	I	Type I-B CRISPR-associated protein Cas8	polymer	534	63474.3	1	UniProt (A0A235IGR9) In PDB	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
5	J, K, L	Type I-B CRISPR-associated protein Cas11	polymer	138	16314.4	3	UniProt (A0A235IGR9) In PDB	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
6	M	RNA	polymer	71	22876.5	1		synthetic construct
7	N	Target DNA strand	polymer	65	19913.8	1		synthetic construct
8	O	Non-target DNA strand	polymer	65	20150.0	1		synthetic construct

Sequence modifications

I: 3 - 526 (UniProt: A0A235IGR9)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ile 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	448950.3
All*	Total formula weight	448950.3

*Water molecules are not included.