8FDU

Engineered human dynein motor domain in the microtubule-unbound state with LIS1 complex in the buffer containing ATP-Vi (local refined on AAA3-AAA5 and LIS1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cytoplasmic dynein 1 heavy chain 1,Serine--tRNA ligase	polymer	3126	357459.1	1	UniProt (Q14204) UniProt (Q5SJX7) Pfam (PF08393) Pfam (PF03028) Pfam (PF18198) Pfam (PF18199) Pfam (PF12774) Pfam (PF17852) Pfam (PF12775) Pfam (PF22597) Pfam (PF12780) Pfam (PF12777) Pfam (PF02403) Pfam (PF12781) In PDB	Homo sapiens (human)	Cytoplasmic dynein heavy chain 1,Dynein heavy chain,cytosolic,Seryl-tRNA synthetase,SerRS,Seryl-tRNA(Ser/Sec) synthetase
2	B, C	Platelet-activating factor acetylhydrolase IB subunit beta,Platelet-activating factor acetylhydrolase IB subunit beta,human LIS1 protein with a SNAP tag	polymer	598	66423.5	2	UniProt (P43034) Pfam (PF08513) Pfam (PF00400) In PDB	Homo sapiens (human)	Lissencephaly-1 protein,LIS-1,PAF acetylhydrolase 45 kDa subunit,PAF-AH 45 kDa subunit,PAF-AH alpha,PAFAH alpha
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A: 3 - 1822 (UniProt: Q14204)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag

A: 1823 - 1889 (UniProt: Q5SJX7)
A: 1890 - 3124 (UniProt: Q14204)

PDB	External Database	Details
Glu 3125	-	expression tag
Phe 3126	-	expression tag

B, C: 3 - 411 (UniProt: P43034)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag

B, C: 412 - 598 (PDB: 8FDU)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	490306.2
Non-Polymers*	Number of molecules	2
	Total formula weight	854.4
All*	Total formula weight	491160.6

*Water molecules are not included.