8FD3

Cryo-EM structure of Cascade-PAM complex in type I-B CAST system

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Type I-B CRISPR-associated protein Cas5	polymer	212	24852.9	1	UniProt (A0A235IG00)	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
2	B (B)	Type I-B CRISPR-associated protein Cas6	polymer	220	24945.7	1	UniProt (A0A235IH92) Pfam (PF09559)	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
3	C, D, E, F, G... (C, D, E, F, G...)	Type I-B CRISPR-associated protein Cas7	polymer	323	37299.0	6	UniProt (A0A235IG15)	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
4	I (I)	Type I-MYXAN CRISPR-associated Cas8a1/Cmx1	polymer	534	63474.3	1	UniProt (A0A235IGR9)	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
5	J, K, L (J, K, L)	Type I-B CRISPR-associated protein Cas11	polymer	138	16314.4	3	UniProt (A0A235IGR9)	Nostoc sp. 'Peltigera membranacea cyanobiont' 210A
6	M (M)	RNA	polymer	71	22876.5	1		synthetic construct
7	N (N)	Target DNA strand	polymer	10	3004.0	1		synthetic construct
8	O (O)	Non-target DNA strand	polymer	45	13973.0	1		synthetic construct

Sequence modifications

I: 3 - 526 (UniProt: A0A235IGR9)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Ile 2	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	425863.6
All*	Total formula weight	425863.6

*Water molecules are not included.