8FCU
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex in type I-B CAST system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Type I-B CRISPR-associated protein Cas5 | polymer | 212 | 24852.9 | 1 | UniProt (A0A235IG00) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 2 | B (B) | Type I-B CRISPR-associated protein Cas6 | polymer | 220 | 24945.7 | 1 | UniProt (A0A235IH92) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 3 | C, D, E, F, G... (C, D, E, F, G...) | Type I-B CRISPR-associated protein Cas7 | polymer | 323 | 37299.0 | 6 | UniProt (A0A235IG15) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 4 | I (I) | Type I-MYXAN CRISPR-associated Cas8a1/Cmx1 | polymer | 534 | 63474.3 | 1 | UniProt (A0A235IGR9) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 5 | J, K, L (J, K, L) | Type I-B CRISPR-associated protein Cas11 | polymer | 138 | 16314.4 | 3 | UniProt (A0A235IGR9) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 6 | M (M) | RNA | polymer | 71 | 22876.5 | 1 | synthetic construct | ||
| 7 | N (N) | Target DNA strand | polymer | 85 | 26108.7 | 1 | synthetic construct | ||
| 8 | O (O) | Non-target DNA strand | polymer | 85 | 26313.9 | 1 | synthetic construct | ||
| 9 | P (P) | TniQ | polymer | 329 | 37592.0 | 1 | UniProt (A0A235IFP5) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 10 | Q (Q) | TnsC | polymer | 383 | 43521.9 | 1 | UniProt (A0A235IFM2) | Nostoc sp. 'Peltigera membranacea cyanobiont' 210A | |
| 11 | R (Q) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 12 | S (Q) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
I: 3 - 526 (UniProt: A0A235IGR9)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ile 2 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 17 |
| Total formula weight | 542423.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 531.5 | |
| All* | Total formula weight | 542954.6 |
