8FCK

Structure of the vertebrate augmin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	HAUS augmin-like complex subunit 1	polymer	286	32655.6	1	UniProt (A0A8J1L9M8) In PDB	Xenopus laevis (African clawed frog)
2	B	HAUS augmin-like complex subunit 3	polymer	597	68112.3	1	UniProt (Q6DCY9) Pfam (PF14932) In PDB	Xenopus laevis (African clawed frog)
3	C	HAUS augmin like complex subunit 4 L homeolog	polymer	353	41256.4	1	UniProt (Q4V7I1) Pfam (PF14735) In PDB	Xenopus laevis (African clawed frog)	MGC115689 protein
4	D	HAUS augmin-like complex subunit 5	polymer	666	77357.3	1	UniProt (A0A1L8FPI2) Pfam (PF14817) In PDB	Xenopus laevis (African clawed frog)
5	E	HAUS augmin like complex subunit 2 L homeolog, Green fluorescent protein chimera	polymer	472	53505.5	1	UniProt (Q6INL9) UniProt (P42212) Pfam (PF15003) Pfam (PF01353) In PDB	Xenopus laevis (African clawed frog)	MGC82377 protein
6	F	HAUS augmin like complex subunit 6 L homeolog	polymer	442	50928.0	1	UniProt (A0JPI0) Pfam (PF14661) In PDB	Xenopus laevis (African clawed frog)
7	G	HAUS augmin like complex subunit 7 S homeolog	polymer	348	39379.6	1	UniProt (B1H1T5) In PDB	Xenopus laevis (African clawed frog)	LOC100158301 protein
8	H	HAUS augmin-like complex subunit 8	polymer	367	41144.9	1	UniProt (Q0IHJ3) In PDB	Xenopus laevis (African clawed frog)	HEC1/NDC80-interacting centrosome-associated protein 1,Sarcoma antigen NY-SAR-48 homolog

Sequence modifications

A: 1 - 286 (UniProt: A0A8J1L9M8)

PDB	External Database	Details
Gln 156	Arg 156	conflict

E: 1 - 222 (UniProt: Q6INL9)

PDB	External Database	Details
Val 223	-	linker
Asp 224	-	linker
Met 225	-	linker
Val 226	-	linker

E: 227 - 463 (UniProt: P42212)

PDB	External Database	Details
Leu 289	Phe 64	conflict
Thr 290	Ser 65	conflict
Lys 446	Leu 221	conflict
Leu 456	His 231	conflict
Val 464	-	expression tag
Asp 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag
His 469	-	expression tag
His 470	-	expression tag
His 471	-	expression tag
His 472	-	expression tag

F: 1 - 430 (UniProt: A0JPI0)

PDB	External Database	Details
Gly -11	-	expression tag
Pro -10	-	expression tag
Ala -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Thr -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ala -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	404339.5
All*	Total formula weight	404339.5

*Water molecules are not included.