8F7R

Gi bound mu-opioid receptor in complex with endomorphin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R, M	Mu-type opioid receptor	polymer	403	45479.7	2	UniProt (P35372) Pfam (PF00001) In PDB	Homo sapiens (human)	M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
2	P, Q	endomorphin	polymer	5	609.7	2		Homo sapiens
3	A, F	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	2	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
5	C	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
6	E	scFv16	polymer	248	26408.5	1		synthetic construct
7	R, M	CHOLESTEROL	non-polymer		386.7	10	Chemie (CLR)

Sequence modifications

R, M: 2 - 388 (UniProt: P35372)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Val 1	-	expression tag
His 389	-	expression tag
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag
His 396	-	expression tag

A, F: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	246061.8
Non-Polymers*	Number of molecules	10
	Total formula weight	3866.5
All*	Total formula weight	249928.3

*Water molecules are not included.