8F6X
cryo-EM structure of a structurally designed Human metapneumovirus F protein in complex with antibody MPE8
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Structurally designed HMPV F protein HMPV_v3B_D12_DS454,Fibritin | polymer | 522 | 56891.6 | 3 | UniProt (G3KCK8) UniProt (A0A2Z5WL46) Pfam (PF00523) UniProt (by SIFTS) (P10104) | Human metapneumovirus | |
| 2 | D, E, F (D, E, F) | MPE8 Single chain variable fragment | polymer | 306 | 32012.1 | 3 | Homo sapiens | ||
| 3 | G, H, I (A, B, C) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) |
Sequence modifications
A, B, C: 1 - 88 (UniProt: G3KCK8)
A, B, C: 113 - 485 (UniProt: G3KCK8)
A, B, C: 490 - 516 (UniProt: A0A2Z5WL46)
| PDB | External Database | Details |
|---|---|---|
| Cys 84 | Val 84 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 89 | - | linker |
| Ser 90 | - | linker |
| Gly 91 | - | linker |
| Gly 92 | - | linker |
| Ser 93 | - | linker |
| Gly 94 | - | linker |
| Cys 140 | Ala 140 | engineered mutation |
| Cys 147 | Ala 147 | engineered mutation |
| Pro 185 | Ala 185 | engineered mutation |
| Cys 249 | Ala 249 | engineered mutation |
| Cys 454 | Asp 454 | engineered mutation |
| Cys 458 | Val 458 | engineered mutation |
| Ser 486 | - | linker |
| Ala 487 | - | linker |
| Ile 488 | - | linker |
| Gly 489 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 517 | - | expression tag |
| Gly 518 | - | expression tag |
| Leu 519 | - | expression tag |
| Val 520 | - | expression tag |
| Pro 521 | - | expression tag |
| Arg 522 | - | expression tag |
| Gly 523 | - | expression tag |
| Ser 524 | - | expression tag |
| His 525 | - | expression tag |
| His 526 | - | expression tag |
| His 527 | - | expression tag |
| His 528 | - | expression tag |
| His 529 | - | expression tag |
| His 530 | - | expression tag |
| Ser 531 | - | expression tag |
| Ala 532 | - | expression tag |
| Trp 533 | - | expression tag |
| Ser 534 | - | expression tag |
| His 535 | - | expression tag |
| Pro 536 | - | expression tag |
| Gln 537 | - | expression tag |
| Phe 538 | - | expression tag |
| Glu 539 | - | expression tag |
| Lys 540 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 266711.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 663.6 | |
| All* | Total formula weight | 267374.7 |
