8F4C

RT XFEL structure of the two-flash state of Photosystem II (2F, S3-rich) at 2.00 Angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, a	Photosystem II protein D1 1	polymer	344	38265.6	2	UniProt (P0A444) Pfam (PF00124) In PDB	Thermosynechococcus vestitus BP-1	PSII D1 protein 1,Photosystem II Q(B) protein 1
2	B, b	Photosystem II CP47 reaction center protein	polymer	510	56656.5	2	UniProt (Q8DIQ1) Pfam (PF00421) In PDB	Thermosynechococcus vestitus BP-1	PSII 47 kDa protein,Protein CP-47
3	C, c	Photosystem II CP43 reaction center protein	polymer	461	50287.5	2	UniProt (Q8DIF8) Pfam (PF00421) In PDB	Thermosynechococcus vestitus BP-1	PSII 43 kDa protein,Protein CP-43
4	D, d	Photosystem II D2 protein	polymer	352	39388.2	2	UniProt (Q8CM25) Pfam (PF00124) In PDB	Thermosynechococcus vestitus BP-1	PSII D2 protein,Photosystem II Q(A) protein
5	E, e	Cytochrome b559 subunit alpha	polymer	84	9580.8	2	UniProt (Q8DIP0) Pfam (PF00283) Pfam (PF00284) In PDB	Thermosynechococcus vestitus BP-1	PSII reaction center subunit V
6	F, f	Cytochrome b559 subunit beta	polymer	45	5067.9	2	UniProt (Q8DIN9) Pfam (PF00283) In PDB	Thermosynechococcus vestitus BP-1	PSII reaction center subunit VI
7	H, h	Photosystem II reaction center protein H	polymer	66	7358.8	2	UniProt (Q8DJ43) Pfam (PF00737) In PDB	Thermosynechococcus vestitus BP-1	PSII-H
8	I, i	Photosystem II reaction center protein I	polymer	38	4438.3	2	UniProt (Q8DJZ6) Pfam (PF02532) In PDB	Thermosynechococcus vestitus BP-1	PSII-I,PSII 4.4 kDa protein
9	J, j	Photosystem II reaction center protein J	polymer	40	4105.9	2	UniProt (P59087) Pfam (PF01788) In PDB	Thermosynechococcus vestitus BP-1	PSII-J
10	K, k	Photosystem II reaction center protein K	polymer	46	5028.1	2	UniProt (Q9F1K9) Pfam (PF02533) In PDB	Thermosynechococcus vestitus BP-1	PSII-K
11	L, l	Photosystem II reaction center protein L	polymer	37	4299.0	2	UniProt (Q8DIN8) Pfam (PF02419) In PDB	Thermosynechococcus vestitus BP-1	PSII-L
12	M, m	Photosystem II reaction center protein M	polymer	36	4009.7	2	UniProt (Q8DHA7) Pfam (PF05151) In PDB	Thermosynechococcus vestitus BP-1	PSII-M
13	O, o	Photosystem II manganese-stabilizing polypeptide	polymer	272	29637.4	2	UniProt (P0A431) Pfam (PF01716) In PDB	Thermosynechococcus vestitus BP-1	MSP
14	T, t	Photosystem II reaction center protein T	polymer	32	3906.7	2	UniProt (Q8DIQ0) Pfam (PF01405) In PDB	Thermosynechococcus vestitus BP-1	PSII-Tc
15	U, u	Photosystem II 12 kDa extrinsic protein	polymer	134	15031.0	2	UniProt (Q9F1L5) Pfam (PF06514) In PDB	Thermosynechococcus vestitus BP-1	PS II complex 12 kDa extrinsic protein,PSII-U
16	V, v	Cytochrome c-550	polymer	163	18046.9	2	UniProt (P0A386) Pfam (PF14495) In PDB	Thermosynechococcus vestitus BP-1	Cytochrome c-549,Cytochrome c550,Low-potential cytochrome c
17	Y, y	Photosystem II reaction center protein Ycf12	polymer	46	5039.1	2	UniProt (Q8DJI1) Pfam (PF05969) In PDB	Thermosynechococcus vestitus BP-1
18	X, x	Photosystem II reaction center X protein	polymer	41	4322.2	2	UniProt (Q9F1R6) Pfam (PF06596) In PDB	Thermosynechococcus vestitus BP-1
19	Z, z	Photosystem II reaction center protein Z	polymer	62	6766.2	2	UniProt (Q8DHJ2) Pfam (PF01737) In PDB	Thermosynechococcus vestitus BP-1	PSII-Z
20	R, r	Photosystem II protein Y	polymer	41	4590.6	2	UniProt (Q8DKM3) Pfam (PF06298) In PDB	Thermosynechococcus vestitus BP-1
21	a, A	CA-MN4-O6 CLUSTER	non-polymer		355.8	2	Chemie (OEY)
22	a, A	CA-MN4-O5 CLUSTER	non-polymer		339.8	2	Chemie (OEX)
23	a, A	FE (II) ION	non-polymer		55.8	2	Chemie (FE2)
24	a, A	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
25	b, c, D, B, C...	CHLOROPHYLL A	non-polymer		893.5	70	Chemie (CLA)
26	Z, D, c, k, b...	BETA-CAROTENE	non-polymer		536.9	22	Chemie (BCR)
27	D, a, d, A	2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE	non-polymer		749.2	4	Chemie (PL9)
28	d, D, c, b, C...	1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE	non-polymer		787.2	13	Chemie (LMG)
29	A, e, D, E, l...	1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE	non-polymer		723.0	10	Chemie (LHG)
30	B, A, f, D, b...	1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL	non-polymer		795.1	8	Chemie (SQD)
31	A, h, c, o, H...	DIGALACTOSYL DIACYL GLYCEROL (DGDG)	non-polymer		949.3	10	Chemie (DGD)
32	T, B, d, X, j...	STEARIC ACID	non-polymer		284.5	31	Chemie (STE)
33	D, a	BICARBONATE ION	non-polymer		61.0	2	Chemie (BCT)
34	D, d	PHEOPHYTIN A	non-polymer		871.2	4	Chemie (PHO)
35	f, F	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
36	V, v	HEME C	non-polymer		618.5	2	Chemie (HEC)
37		water	water		18.0	2020	Chemie (HOH)

Sequence modifications

I, i: 2 - 38 (UniProt: Q8DJZ6)

PDB	External Database	Details
Fme 1	-	initiating methionine

T, t: 2 - 32 (UniProt: Q8DIQ0)

PDB	External Database	Details
Fme 1	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	40
	Total formula weight	631653.0
Non-Polymers*	Number of molecules	188
	Total formula weight	127209.3
All*	Total formula weight	758862.4

*Water molecules are not included.