8F3C
Cryo-EM consensus structure of Escherichia coli que-PEC (paused elongation complex) RNA Polymerase minus preQ1 ligand
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (38-MER) | polymer | 39 | 12063.8 | 1 | Escherichia coli | ||
| 2 | B (B) | DNA (39-MER) | polymer | 39 | 11917.6 | 1 | Escherichia coli | ||
| 3 | C, D (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 328 | 36429.6 | 2 | UniProt (A1AGI6) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | E (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | F (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1434 | 158603.3 | 1 | UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | |
| 6 | G (K) | DNA-directed RNA polymerase subunit omega | polymer | 102 | 11519.9 | 1 | UniProt (A1AHI0) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | H (R) | RNA (47-MER) | polymer | 47 | 15059.1 | 1 | Escherichia coli | ||
| 8 | I (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
J: 1 - 1407 (UniProt: C3SIA2)
K: 1 - 91 (UniProt: A1AHI0)
| PDB | External Database | Details |
|---|---|---|
| Leu 1408 | - | expression tag |
| Asp 1409 | - | expression tag |
| Arg 1410 | - | expression tag |
| Arg 1411 | - | expression tag |
| Ala 1412 | - | expression tag |
| Ser 1413 | - | expression tag |
| Glu 1414 | - | expression tag |
| Asn 1415 | - | expression tag |
| Leu 1416 | - | expression tag |
| Tyr 1417 | - | expression tag |
| Phe 1418 | - | expression tag |
| Gln 1419 | - | expression tag |
| Gly 1420 | - | expression tag |
| Gly 1421 | - | expression tag |
| Leu 1422 | - | expression tag |
| Asn 1423 | - | expression tag |
| Asp 1424 | - | expression tag |
| Ile 1425 | - | expression tag |
| Phe 1426 | - | expression tag |
| Glu 1427 | - | expression tag |
| Ala 1428 | - | expression tag |
| Gln 1429 | - | expression tag |
| Lys 1430 | - | expression tag |
| Ile 1431 | - | expression tag |
| Glu 1432 | - | expression tag |
| Trp 1433 | - | expression tag |
| His 1434 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 92 | - | expression tag |
| Ala 93 | - | expression tag |
| Ala 94 | - | expression tag |
| Val 95 | - | expression tag |
| Glu 96 | - | expression tag |
| His 97 | - | expression tag |
| His 98 | - | expression tag |
| His 99 | - | expression tag |
| His 100 | - | expression tag |
| His 101 | - | expression tag |
| His 102 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 432843.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 432868.0 |
