8F1J

SigN RNA polymerase early-melted intermediate bound to mismatch DNA fragment dhsU36mm2 (-12A)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	DNA (36-MER)	polymer	36	11071.2	2	GenBank (AE000657.1)	Aquifex aeolicus
2	G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
3	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
4	J	DNA-directed RNA polymerase subunit beta'	polymer	1430	158105.7	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
5	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
6	M	RNA polymerase sigma-54 factor	polymer	480	54406.9	1	UniProt (P24255) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) In PDB	Escherichia coli
7	B, D	DNA (36-MER)	polymer	36	11054.1	2	GenBank (AE000657.1)	Aquifex aeolicus
8	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
9	J	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10		water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, C: 1 - 36 (GenBank: AE000657.1)

PDB	External Database	Details
Dc 24	Da 144241	engineered mutation
Dt 25	Da 144242	engineered mutation

J: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag
Gly 1416	-	expression tag
Pro 1417	-	expression tag
Ser 1418	-	expression tag
Ser 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

M: 1 - 477 (UniProt: P24255)

PDB	External Database	Details
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

B, D: 37 - 72 (GenBank: AE000657.1)

PDB	External Database	Details
Da 49	Dt 144241	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	490950.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	491106.1

*Water molecules are not included.