8EZJ

Cryo-EM structure of the S. cerevisiae Arf-like protein Arl1 bound to the Arf guanine nucleotide exchange factor Gea2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ARF guanine-nucleotide exchange factor 2	polymer	1483	168590.7	2	UniProt (P39993) Pfam (PF12783) Pfam (PF01369) In PDB	Saccharomyces cerevisiae (baker's yeast)
2	C, D	ADP-ribosylation factor-like protein 1	polymer	183	20460.2	2	UniProt (P38116) Pfam (PF00025) In PDB	Saccharomyces cerevisiae (baker's yeast)	Arf-like GTPase 1
3	C, D	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)

Sequence modifications

A, B: 1 - 1459 (UniProt: P39993)

PDB	External Database	Details
Met -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

C, D: 18 - 183 (UniProt: P38116)

PDB	External Database	Details
Leu 72	Gln 72	engineered mutation
Ala 184	-	expression tag
Gly 185	-	expression tag
Glu 186	-	expression tag
Asn 187	-	expression tag
Leu 188	-	expression tag
Tyr 189	-	expression tag
Phe 190	-	expression tag
Gln 191	-	expression tag
Ser 192	-	expression tag
Ala 193	-	expression tag
Gly 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	378101.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1046.4
All*	Total formula weight	379148.1

*Water molecules are not included.