8EZJ
Cryo-EM structure of the S. cerevisiae Arf-like protein Arl1 bound to the Arf guanine nucleotide exchange factor Gea2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | ARF guanine-nucleotide exchange factor 2 | polymer | 1483 | 168590.7 | 2 | UniProt (P39993) Pfam (PF12783) Pfam (PF01369) | Saccharomyces cerevisiae (baker's yeast) | |
| 2 | C, D (C, D) | ADP-ribosylation factor-like protein 1 | polymer | 183 | 20460.2 | 2 | UniProt (P38116) Pfam (PF00025) | Saccharomyces cerevisiae (baker's yeast) | Arf-like GTPase 1 |
| 3 | E, F (C, D) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
A, B: 1 - 1459 (UniProt: P39993)
C, D: 18 - 183 (UniProt: P38116)
| PDB | External Database | Details |
|---|---|---|
| Met -23 | - | initiating methionine |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Val -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 72 | Gln 72 | engineered mutation |
| Ala 184 | - | expression tag |
| Gly 185 | - | expression tag |
| Glu 186 | - | expression tag |
| Asn 187 | - | expression tag |
| Leu 188 | - | expression tag |
| Tyr 189 | - | expression tag |
| Phe 190 | - | expression tag |
| Gln 191 | - | expression tag |
| Ser 192 | - | expression tag |
| Ala 193 | - | expression tag |
| Gly 194 | - | expression tag |
| His 195 | - | expression tag |
| His 196 | - | expression tag |
| His 197 | - | expression tag |
| His 198 | - | expression tag |
| His 199 | - | expression tag |
| His 200 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 378101.7 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1046.4 | |
| All* | Total formula weight | 379148.1 |
