8EYZ

Engineered glutamine binding protein bound to GLN and a cobaloxime ligand

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Amino acid ABC transporter substrate-binding protein	polymer	229	25254.7	12	UniProt (C3THM2) Pfam (PF00497) UniProt (by SIFTS) (P0AEQ3) In PDB	Escherichia coli	Glutamine ABC transporter substrate-binding protein GlnH,Glutamine ABC transporter substrate-bindnig protein,Glutamine ABC transporter,periplasmic glutamine-binding protein (TC 3.A.1.3.2),substrate-binding protein,Glutamine-binding periplasmic protein,Periplasmic glutamine-binding protein,Periplasmic glutamine-binding protein permease,Permease of periplasmic glutamine-binding protein
2	E, F, G, H, A...	AZIDOBIS (DIMETHYLGLYOXIMATO) PYRIDINECOBALT	non-polymer		438.3	12	Chemie (X5Z)
3	G, A, J, B, C	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4	E, F, G, H, I...	GLUTAMINE	non-polymer		146.1	12	Chemie (GLN)
5	F, D	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6		water	water		18.0	87	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 226 (UniProt: C3THM2)

PDB	External Database	Details
Ser -3	-	expression tag
Asn -2	-	expression tag
Ala -1	-	expression tag
Ile 23	Lys 45	engineered mutation
Cys 72	Thr 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	303055.9
Non-Polymers*	Number of molecules	35
	Total formula weight	8058.4
All*	Total formula weight	311114.3

*Water molecules are not included.