8EYA
Crystal structure of PTP1B D181A/Q262A/C215A phosphatase domain with a JAK2 activation loop phosphopeptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Tyrosine-protein phosphatase non-receptor type 1 | polymer | 302 | 34997.9 | 2 | UniProt (P18031) Pfam (PF00102) | Homo sapiens (human) | Protein-tyrosine phosphatase 1B,PTP-1B |
| 2 | C, D (D, E) | Tyrosine-protein kinase JAK2 activation loop phosphopeptide | polymer | 16 | 2085.1 | 2 | UniProt (O60674) | Homo sapiens | Janus kinase 2,JAK-2 |
| 3 | E (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 4 | F, L (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 5 | G, H, I, M (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 6 | J, K, N (A, B) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 7 | O, P, Q, R (A, B, D, E) | water | water | 18.0 | 175 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 301 (UniProt: P18031)
| PDB | External Database | Details |
|---|---|---|
| Ala 181 | Asp 181 | engineered mutation |
| Ala 215 | Cys 215 | engineered mutation |
| Ala 262 | Gln 262 | engineered mutation |
| Ala 302 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 74166.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 457.1 | |
| All* | Total formula weight | 74623.1 |
