8EUO

Hydroxynitrile Lyase from Hevea brasiliensis with Seven Mutations

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	(S)-hydroxynitrile lyase	polymer	265	30258.7	1	UniProt (P52704) Pfam (PF00561)	Hevea brasiliensis (rubber tree)	(S)-acetone-cyanohydrin lyase,Oxynitrilase
2	B (A)	water	water		18.0	132	Chemie (HOH)

Sequence modifications

A: 1 - 257 (UniProt: P52704)

PDB	External Database	Details
Gly 11	Thr 11	engineered mutation
His 79	Glu 79	engineered mutation
Leu 81	Cys 81	engineered mutation
Val 103	His 103	engineered mutation
Ala 104	Asn 104	engineered mutation
Ser 176	Gly 176	engineered mutation
Met 236	Lys 236	engineered mutation
Leu 258	-	expression tag
Glu 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30258.7
All*	Total formula weight	30258.7

*Water molecules are not included.