8EUM

MicroED structure of an Aeropyrum pernix protoglobin mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Protogloblin ApPgb	polymer	190	22187.3	4	UniProt (Q9YFF4) Pfam (PF11563) In PDB	Aeropyrum pernix
2	A, B, C, D	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)
3	A, B, C, D	IMIDAZOLE	non-polymer		69.1	15	Chemie (IMD)
4	D, A, B, C	FE (III) ION	non-polymer		55.8	4	Chemie (FE)
5		water	water		18.0	308	Chemie (HOH)

Sequence modifications

A, B, C, D: 6 - 195 (UniProt: Q9YFF4)

PDB	External Database	Details
Gly 45	Cys 45	engineered mutation
Leu 59	Trp 59	engineered mutation
Val 60	Tyr 60	engineered mutation
Arg 63	Val 63	engineered mutation
Ser 102	Cys 102	engineered mutation
Gln 145	Phe 145	engineered mutation
Leu 149	Ile 149	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88749.2
Non-Polymers*	Number of molecules	23
	Total formula weight	3725.6
All*	Total formula weight	92474.8

*Water molecules are not included.