8EU8

Cryo-EM structure of CH848 10.17DT DS-SOSIP-2P Env

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	CH848 10.17DT SOSIP Envelope glycoprotein gp160	polymer	698	77695.1	3	UniProt (A0A1W6IPB2) Pfam (PF00516) Pfam (PF00517)	Human immunodeficiency virus 1	Env polyprotein, CH848 10.17DT DS-SOSIP-2P
2	D, E, F, G, H... (D, E, F, G, H...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	18	In PDB GlyTouCan (G42666HT)
3	AA, BA, CA, DA, EA... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	27	Chemie (NAG)

Sequence modifications

A, B, C: 34 - 664 (UniProt: A0A1W6IPB2)

PDB	External Database	Details
Met 7	-	initiating methionine
Pro 8	-	expression tag
Met 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Leu 12	-	expression tag
Gln 13	-	expression tag
Pro 14	-	expression tag
Leu 15	-	expression tag
Ala 16	-	expression tag
Thr 17	-	expression tag
Leu 18	-	expression tag
Tyr 19	-	expression tag
Leu 20	-	expression tag
Leu 21	-	expression tag
Gly 22	-	expression tag
Met 23	-	expression tag
Leu 24	-	expression tag
Val 25	-	expression tag
Ala 26	-	expression tag
Ser 27	-	expression tag
Val 28	-	expression tag
Leu 29	-	expression tag
Ala 30	-	expression tag
Ala 31	-	expression tag
Glu 32	-	expression tag
Asn 33	-	expression tag
Asp 133	Asn 129	conflict
Thr 138	Asn 134	conflict
Cys 201	Val 189	conflict
Cys 433	Ala 417	conflict
Lys 490	Glu 474	conflict
Glu 492	Gln 476	conflict
Val 496	Ile 480	conflict
Arg 500	Gly 484	conflict
Cys 501	Ala 485	conflict
Gly 505	-	insertion
Arg 505	-	insertion
Arg 505	Glu 491	conflict
Arg 505	Glu 493	conflict
Arg 505	Lys 494	conflict
Val 505	Ala 497	conflict
Ile 505	Leu 499	conflict
Val 505	Leu 502	conflict
Met 535	Ile 519	conflict
Asn 543	Gln 527	conflict
Pro 559	Ile 543	engineered mutation
Leu 565	Met 549	conflict
Lys 567	Gln 551	conflict
Pro 568	Leu 552	conflict
Pro 569	Thr 553	conflict
Val 583	Leu 567	conflict
Arg 588	Lys 572	conflict
Ile 595	Met 579	conflict
Cys 605	Thr 589	conflict
Ser 612	Thr 596	conflict
Arg 617	Lys 601	conflict
Asn 618	Ser 602	conflict
Leu 619	Glu 603	conflict
Ser 620	Thr 604	conflict
Glu 621	Asp 605	conflict
Leu 629	Met 613	conflict
Asp 632	Glu 616	conflict
Lys 633	Arg 617	conflict
Gln 640	Glu 624	conflict
Ile 641	Thr 625	conflict
Gly 644	Lys 628	conflict
Glu 648	Asp 632	conflict
Lys 655	Arg 639	conflict
Gly 665	-	expression tag
Ser 666	-	expression tag
Leu 667	-	expression tag
Glu 668	-	expression tag
Val 669	-	expression tag
Leu 670	-	expression tag
Phe 671	-	expression tag
Gln 672	-	expression tag
Gly 673	-	expression tag
Pro 674	-	expression tag
Gly 675	-	expression tag
His 676	-	expression tag
His 677	-	expression tag
His 678	-	expression tag
His 679	-	expression tag
His 680	-	expression tag
His 681	-	expression tag
His 682	-	expression tag
His 683	-	expression tag
Ser 684	-	expression tag
Ala 685	-	expression tag
Trp 686	-	expression tag
Ser 687	-	expression tag
His 688	-	expression tag
Pro 689	-	expression tag
Gln 690	-	expression tag
Phe 691	-	expression tag
Glu 692	-	expression tag
Lys 693	-	expression tag
Gly 694	-	expression tag
Gly 695	-	expression tag
Gly 696	-	expression tag
Ser 697	-	expression tag
Gly 698	-	expression tag
Gly 699	-	expression tag
Gly 700	-	expression tag
Gly 701	-	expression tag
Ser 702	-	expression tag
Gly 703	-	expression tag
Gly 704	-	expression tag
Ser 705	-	expression tag
Ala 706	-	expression tag
Trp 707	-	expression tag
Ser 708	-	expression tag
His 709	-	expression tag
Pro 710	-	expression tag
Gln 711	-	expression tag
Phe 712	-	expression tag
Glu 713	-	expression tag
Lys 714	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	233085.2
Branched	Number of molecules	18
	Total formula weight	7639.2
Non-Polymers*	Number of molecules	27
	Total formula weight	5972.6
All*	Total formula weight	246697.0

*Water molecules are not included.