8EQU

Structure of SARS-CoV-2 Orf3a in late endosome/lysosome-like environment, Saposin A nanodisc

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ORF3a protein	polymer	331	36489.4	2	UniProt (P0DTC3) Pfam (PF11289)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	ORF3a,Accessory protein 3a,Protein 3a,Protein U274,Protein X1
2	C, F (C, F)	Saposin-A	polymer	106	11677.3	2	UniProt (P07602) Pfam (PF05184) Pfam (PF03489)	Homo sapiens (human)	Protein A
3	D, E, G, H (D, E, G, H)	Saposin A, polyalanine model	polymer	79	6741.3	4		Homo sapiens
4	I, J (A, B)	1,2-dioleoyl-sn-glycero-3-phosphoethanolamine	non-polymer		744.0	2	Chemie (PEE)

Sequence modifications

A, B: 1 - 275 (UniProt: P0DTC3)

PDB	External Database	Details
Gly 276	-	expression tag
Gly 277	-	expression tag
Arg 278	-	expression tag
Gly 279	-	expression tag
Leu 280	-	expression tag
Glu 281	-	expression tag
Val 282	-	expression tag
Leu 283	-	expression tag
Phe 284	-	expression tag
Gln 285	-	expression tag
Gly 286	-	expression tag
Pro 287	-	expression tag
Gly 288	-	expression tag
Ser 289	-	expression tag
Gly 290	-	expression tag
Gln 291	-	expression tag
Leu 292	-	expression tag
Val 293	-	expression tag
Gly 294	-	expression tag
Ser 295	-	expression tag
Gly 296	-	expression tag
Gly 297	-	expression tag
Leu 298	-	expression tag
Glu 299	-	expression tag
Gly 300	-	expression tag
Gly 301	-	expression tag
Gly 302	-	expression tag
Gly 303	-	expression tag
Trp 304	-	expression tag
Ser 305	-	expression tag
His 306	-	expression tag
Pro 307	-	expression tag
Gln 308	-	expression tag
Phe 309	-	expression tag
Glu 310	-	expression tag
Lys 311	-	expression tag
Gly 312	-	expression tag
Gly 313	-	expression tag
Gly 314	-	expression tag
Ser 315	-	expression tag
Gly 316	-	expression tag
Gly 317	-	expression tag
Gly 318	-	expression tag
Ser 319	-	expression tag
Gly 320	-	expression tag
Gly 321	-	expression tag
Gly 322	-	expression tag
Ser 323	-	expression tag
Trp 324	-	expression tag
Ser 325	-	expression tag
His 326	-	expression tag
Pro 327	-	expression tag
Gln 328	-	expression tag
Phe 329	-	expression tag
Glu 330	-	expression tag
Lys 331	-	expression tag

C, F: 1 - 81 (UniProt: P07602)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Gly -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	123298.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1488.1
All*	Total formula weight	124786.7

*Water molecules are not included.