8EQB

FAM46C/BCCIPalpha/Nanobody complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J	Synthetic nanobody 1	polymer	125	13719.4	4		Lama glama (llama)
2	B, E, H, K	Terminal nucleotidyltransferase 5C	polymer	224	24533.3	4	UniProt (Q5VWP2) Pfam (PF07984) In PDB	Homo sapiens (human)	Non-canonical poly(A) polymerase FAM46C
3	C, F, I, L	Isoform 2 of BRCA2 and CDKN1A-interacting protein	polymer	254	28190.1	4	UniProt (Q9P287-2) Pfam (PF13862) UniProt (by SIFTS) (Q9P287) In PDB	Homo sapiens (human)	P21- and CDK-associated protein 1,Protein TOK-1

Sequence modifications

B, E, H, K: 15 - 214 (UniProt: Q5VWP2)

PDB	External Database	Details
Gly 10	-	expression tag
Pro 11	-	expression tag
Gly 12	-	expression tag
Ala 13	-	expression tag
Pro 14	-	expression tag
Gln 166	Glu 166	engineered mutation

B, E, H, K: 260 - 278 (PDB: 8EQB)
C, F, I, L: 50 - 322 (UniProt: Q9P287-2)

PDB	External Database	Details
Gly 45	-	expression tag
Pro 46	-	expression tag
Gly 47	-	expression tag
Ala 48	-	expression tag
Pro 49	-	expression tag
-	Ser 112	deletion
-	Glu 113	deletion
-	Asp 114	deletion
-	Ser 115	deletion
-	Asn 116	deletion
-	Asp 117	deletion
-	Asp 118	deletion
-	Met 119	deletion
-	Asp 120	deletion
-	Glu 121	deletion
-	Asp 122	deletion
-	Lys 235	deletion
-	Lys 236	deletion
-	Lys 237	deletion
-	Pro 238	deletion
-	Ser 239	deletion
-	Asn 240	deletion
-	Lys 241	deletion
-	Lys 242	deletion
-	Lys 243	deletion
-	Arg 273	deletion
-	Gly 274	deletion
-	Leu 275	deletion
-	Glu 276	deletion

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	265770.9
All*	Total formula weight	265770.9

*Water molecules are not included.