8EPQ

Cryo-EM structure of SARS-CoV-2 Spike trimer S2D14 with two RBDs exposed

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1121	123913.3	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2	S glycoprotein,E2,Peplomer protein
2	D, G, H, J, K...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	11	In PDB GlyTouCan (G42666HT)
3	E, F, I, R	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
4	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	32	Chemie (NAG)

Sequence modifications

A, B, C: 27 - 1147 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 614	Asp 614	conflict
Gly 682	Arg 682	conflict
Ser 683	Arg 683	conflict
Ser 685	Arg 685	conflict
Glu 701	Ala 701	conflict
Ile 727	Leu 727	conflict
Gln 732	Thr 732	conflict
Val 734	Thr 734	conflict
Glu 747	Thr 747	conflict
His 768	Thr 768	conflict
Glu 769	Gly 769	conflict
Ala 817	Phe 817	conflict
Gly 836	Gln 836	conflict
Ala 872	Gln 872	conflict
Ala 884	Ser 884	conflict
Lys 929	Ser 929	conflict
Gly 937	Ser 937	conflict
Pro 986	Lys 986	conflict
Pro 987	Val 987	conflict
Asn 998	Thr 998	conflict
Ala 1003	Ser 1003	conflict
Asn 1005	Gln 1005	conflict
Glu 1027	Thr 1027	conflict
Thr 1070	Ala 1070	conflict
Tyr 1072	Glu 1072	conflict
Asn 1084	Asp 1084	conflict
Asp 1125	Asn 1125	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	371739.8
Branched	Number of molecules	15
	Total formula weight	7014.6
Non-Polymers*	Number of molecules	32
	Total formula weight	7078.7
All*	Total formula weight	385833.0

*Water molecules are not included.