8EKI

CryoEM structure of the Dsl1 complex bound to SNAREs Sec20 and Use1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein transport protein SEC20	polymer	275	32244.3	1	UniProt (P28791) Pfam (PF03908) In PDB	Saccharomyces cerevisiae S288C
2	B	Protein transport protein USE1	polymer	212	24254.8	1	UniProt (P53146) Pfam (PF09753) In PDB	Saccharomyces cerevisiae S288C	SNARE-like tail-anchored protein 1,Unconventional SNARE in the endoplasmic reticulum protein 1
3	C	Protein transport protein TIP20	polymer	701	81253.1	1	UniProt (P33891) Pfam (PF04437) In PDB	Saccharomyces cerevisiae S288C
4	D	Protein transport protein SEC39	polymer	709	82483.8	1	UniProt (Q12745) Pfam (PF08314) In PDB	Saccharomyces cerevisiae S288C	Dependent on SLY1-20 protein 3
5	E	Protein transport protein DSL1	polymer	783	91445.1	1	UniProt (P53847) Pfam (PF11988) Pfam (PF11989) In PDB	Saccharomyces cerevisiae S288C	Dependent on SLY1-20 protein 1

Sequence modifications

E: 1 - 754 (UniProt: P53847)

PDB	External Database	Details
Met -28	-	initiating methionine
Lys -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Ala -18	-	expression tag
Ala -17	-	expression tag
Gly -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	311681.0
All*	Total formula weight	311681.0

*Water molecules are not included.