8EK7

Crystal structure of Hepes and Mg bound 2,3-diketo-5-methylthiopentyl-1-phosphate enolase-phosphatase from Klebsiella aerogenes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Orotidine 5'-phosphate decarboxylase	polymer	253	27348.5	6	UniProt (A0A0M3H420)	Klebsiella aerogenes	OMP decarboxylase,OMPDCase,OMPdecase
2	G, N, S (A, C, E)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	3	Chemie (EPE)
3	H, I, J, L, O... (A, B, C, E)	MAGNESIUM ION	non-polymer		24.3	10	Chemie (MG)
4	K, M, R, W, X (A, B, C, E, F)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	5	Chemie (MPD)
5	AA, BA, CA, DA, Y... (C, D, E, F, A...)	water	water		18.0	685	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 9 - 249 (UniProt: A0A0M3H420)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Glu 42	Asp 34	conflict
Met 152	Thr 144	conflict
Val 182	Ala 174	conflict
Val 239	Ala 231	conflict
Asn 250	-	expression tag
Lys 251	-	expression tag
Gly 252	-	expression tag
Val 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	164090.7
Non-Polymers*	Number of molecules	18
	Total formula weight	1548.8
All*	Total formula weight	165639.6

*Water molecules are not included.