8EJ3
M. tuberculosis RNAP pause escaped complex with Bacillus subtilis NusG and GMPCPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 347 | 37745.3 | 2 | UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Mycobacterium tuberculosis H37Rv | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1178 | 130018.8 | 1 | UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Mycobacterium tuberculosis H37Rv | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1316 | 146969.0 | 1 | UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Mycobacterium tuberculosis H37Rv | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 110 | 11851.1 | 1 | UniProt (P9WGY5) Pfam (PF01192) | Mycobacterium tuberculosis H37Rv | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (G) | Transcription termination/antitermination protein NusG | polymer | 177 | 20154.1 | 1 | UniProt (Q06795) Pfam (PF02357) Pfam (PF00467) | Bacillus subtilis subsp. subtilis str. 168 | |
| 6 | G (N) | DNA (29-MER) | polymer | 40 | 12248.8 | 1 | synthetic construct | ||
| 7 | H (R) | RNA (5'-R(P*UP*CP*GP*GP*CP*AP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3') | polymer | 30 | 9700.8 | 1 | synthetic RNA | ||
| 8 | I (T) | DNA (32-MER) | polymer | 40 | 12180.8 | 1 | synthetic construct | ||
| 9 | J (C) | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | non-polymer | 521.2 | 1 | Chemie (G2P) | |||
| 10 | K, L (D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | M, N (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 418614.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 700.6 | |
| All* | Total formula weight | 419314.8 |
