8EHQ

Mycobacterium tuberculosis paused transcription complex with Bacillus subtilis NusG

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (P9WGZ1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C	DNA-directed RNA polymerase subunit beta	polymer	1178	130018.8	1	UniProt (P9WGY9) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D	DNA-directed RNA polymerase subunit beta'	polymer	1316	146969.0	1	UniProt (P9WGY7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Mycobacterium tuberculosis H37Rv	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E	DNA-directed RNA polymerase subunit omega	polymer	110	11851.1	1	UniProt (P9WGY5) Pfam (PF01192) In PDB	Mycobacterium tuberculosis H37Rv	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	G	Transcription termination/antitermination protein NusG	polymer	177	20154.1	1	UniProt (Q06795) Pfam (PF02357) Pfam (PF00467) In PDB	Bacillus subtilis subsp. subtilis str. 168
6	T	DNA (38-MER)	polymer	40	12180.8	1		synthetic construct
7	N	DNA (38-MER)	polymer	40	12248.8	1		synthetic construct
8	R	RNA (29-MER)	polymer	30	9700.8	1		synthetic construct
9	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
10	D	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	418614.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	418769.3

*Water molecules are not included.