8EG7
Cryo-EM structure of pre-consensus elemental paused elongation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | non-template DNA | polymer | 32 | 9793.3 | 1 | Escherichia coli | ||
| 2 | B (B) | template DNA | polymer | 32 | 9842.3 | 1 | Escherichia coli | ||
| 3 | C (R) | RNA | polymer | 16 | 5055.0 | 1 | Escherichia coli | ||
| 4 | D, E (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 2 | UniProt (P0A7Z6) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 5 | F (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 6 | G (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155334.7 | 1 | UniProt (C3SIA2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 7 | H (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | I, J (I) | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) PubChem (16402373) PubChem (44119969) PubChem (44660322) PubChem (23636791) PubChem (23639399) PubChem (122146) PubChem (53393747) PubChem (50987332) PubChem (71464051) PubChem (3253739) PubChem (71305076) PubChem (90479263) PubChem (96545191) PubChem (124745618) PubChem (124901558) PubChem (124905421) PubChem (125027410) PubChem (10009199) PubChem (96327541) PubChem (96327542) PubChem (96327543) PubChem (96327544) PubChem (96545192) PubChem (102565431) PubChem (121392376) PubChem (122130148) PubChem (9895487) PubChem (144605089) PubChem (124901556) PubChem (124901561) PubChem (124901563) PubChem (124905422) PubChem (125027411) PubChem (129537355) PubChem (129537356) PubChem (145705472) PubChem (146030291) PubChem (146159852) | |||
| 9 | K (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 10 | L, M (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
G, H: 1 - 234 (UniProt: P0A7Z6)
J: 2 - 1407 (UniProt: C3SIA2)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Val 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 394014.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1418.9 | |
| All* | Total formula weight | 395432.9 |
