8EFQ

DAMGO-bound mu-opioid receptor-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40445.1	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	353	39020.7	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	P	DAMGO	polymer	4	470.5	1		synthetic construct
5	R	Mu-type opioid receptor	polymer	367	41072.2	1	UniProt (P35372) Pfam (PF00001) In PDB	Homo sapiens (human)	M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
6	P	ETHANOLAMINE	non-polymer		61.1	1	Chemie (ETA)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	128572.2
Non-Polymers*	Number of molecules	1
	Total formula weight	61.1
All*	Total formula weight	128633.3

*Water molecules are not included.