8EDG

Cryo-EM structure of the Hermes transposase bound to two left-ends of its DNA transposon

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (M, P)	DNA (5'-D(*GP*CP*GP*TP*GP*AP*A)-3')	polymer	7	2162.4	2		Musca domestica
2	B, D (N, Q)	DNA (46-MER)	polymer	46	14173.1	2		Musca domestica
3	C, E (O, R)	DNA (55-MER)	polymer	55	16931.9	2		Musca domestica
4	G, H, I, J, K... (A, C, I, E, G...)	Hermes transposase	polymer	612	70210.6	6	UniProt (Q25438)	Musca domestica (house fly)
5	M, N, O, P, Q... (A, C, I, E, G...)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, C, I, E, G, K: 1 - 612 (UniProt: Q25438)

PDB	External Database	Details
Glu 2	Gln 2	engineered mutation
Gly 128	Lys 128	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	487798.3
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	488190.8

*Water molecules are not included.