8ECE

E. coli L-asparaginase II mutant (V27T) in complex with L-Glu

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-asparaginase 2	polymer	334	35703.9	4	UniProt (P00805) Pfam (PF00710) Pfam (PF17763)	Escherichia coli K-12	L-asparaginase II,L-ASNase II,L-asparagine amidohydrolase II
2	E, G, J, M (A, B, C, D)	GLUTAMIC ACID	non-polymer		147.1	4	Chemie (GLU)
3	F, H, I, K, L (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
4	N, O, P, Q (A, B, C, D)	water	water		18.0	1186	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 326 (UniProt: P00805)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Thr 27	Val 49	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142815.8
Non-Polymers*	Number of molecules	9
	Total formula weight	898.9
All*	Total formula weight	143714.6

*Water molecules are not included.