8E9M

Crystal structure of E. coli aspartate aminotransferase mutant VFIT bound to maleic acid at 278 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Aspartate aminotransferase	polymer	406	44793.5	2	UniProt (P00509) Pfam (PF00155)	Escherichia coli	AspAT,Transaminase A
2	C, E (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D, F (A, B)	MALEIC ACID	non-polymer		116.1	2	Chemie (MAE)
4	G, H (A, B)	water	water		18.0	354	Chemie (HOH)

Sequence modifications

A, B: 2 - 396 (UniProt: P00509)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Val -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag
Phe 37	Lys 37	engineered mutation
Ile 43	Thr 43	engineered mutation
Thr 64	Asn 64	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89587.0
Non-Polymers*	Number of molecules	4
	Total formula weight	726.4
All*	Total formula weight	90313.4

*Water molecules are not included.