8E9J

Crystal structure of E. coli aspartate aminotransferase mutant HEX in the ligand-free form at 278 K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Aspartate aminotransferase	polymer	406	44794.5	2	UniProt (P00509) Pfam (PF00155) In PDB	Escherichia coli	AspAT,Transaminase A
2	A, B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4		water	water		18.0	244	Chemie (HOH)

Sequence modifications

A, B: 2 - 396 (UniProt: P00509)

PDB	External Database	Details
Met -9	-	initiating methionine
Ala -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Val -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag
Leu 35	Val 35	engineered mutation
Tyr 37	Lys 37	engineered mutation
Ile 43	Thr 43	engineered mutation
Leu 64	Asn 64	engineered mutation
Ser 104	Thr 104	engineered mutation
Ser 285	Asn 285	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89589.1
Non-Polymers*	Number of molecules	4
	Total formula weight	686.4
All*	Total formula weight	90275.5

*Water molecules are not included.